Biochar activation with exoenzymes induced by earthworms: A novel functional strategy for soil quality promotion

• Published in: Journal of hazardous materials 2018-05, Vol.350, p.136-143
• Main Author: Sanchez-Hernandez, Juan C.
• Format: Article
• Language: English
• Subjects: Bioactivation; Biochar; Earthworms; Extracellular enzymes; Soil pollution
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2018.02.019

[Display omitted] •Enzymatic activation of biochar was facilitated in the presence of earthworms.•Biochar-bound enzymes showed a high stability.•Biochar-bound carboxylesterases: novel ligands for binding organophosphate pesticides.•Spent coffee ground-derived biochar retained the highest extracellular enzyme activities. The activation of biochar (carbonaceous material generated by pyrolysis of biomass) with extracellular enzymes generated from soil biological processes was studied via a 2-month microcosm using earthworms. The isolation of biochar particles (post-incubated biochar) from earthworm-treated soils allowed to confirm an enrichment of biochar with extracellular enzymes associated to biogeochemical (alkaline phosphatase, β-glucosidase and arylsulfatase) and bioremediation pathways (carboxylesterase). The hydrolytic activity of this biochar incubated with earthworms was up to 8 times higher compared with that of control biochar (incubated in earthworm-free soils). More interestingly, the enzyme carboxylesterase was stabilized onto biochar surface as demonstrated by its unchanged hydrolytic activity after multiple water rinses of the post-incubated biochar, and the remaining activity following heat stress. Moreover, this biochar-bound carboxylesterase activity was sensitive to inhibition by organophosphorus (23–100% of control activity) and methyl carbamate pesticides (37–57%), demonstrating that bioactivated biochar with carboxylesterases may inactivate these agrochemicals by providing stable ligands of enzymatic nature.