12-OAHSA is a component of olive oil and mitigates obesity-induced inflammation

• Published in: The Journal of nutritional biochemistry 2022-12, Vol.110, p.109127, Article 109127
• Main Authors: Moyo, Knowledge Mudhibadhi, Choi, Jieun, Chang, Jiyeon, Soedono, Shindy, Nguyet, Dan Vo Hoang, Song, Yu-Ri, Park, Soo Jin, Go, Gwang-Woong, Lee, Do Yup, Cho, Kae Won
• Format: Article
• Language: English
• Subjects: 12-OAHSA; Adipose tissue inflammation; Animals; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Corn Oil; FAHFA; Fatty Acids - metabolism; Inflammation - metabolism; Inflammation - prevention & control; LC-MS/MS; Mice; Obesity - metabolism; Oleic Acid; Olive oil; Olive Oil - pharmacology; Stearic Acids
• ISSN: 0955-2863; 1873-4847
• DOI: 10.1016/j.jnutbio.2022.109127

Fatty acid esters of hydroxyl fatty acids (FAHFAs) are a new family of endogenous lipids that exert anti-inflammatory action. Among the various FAHFA isomers, the dietary source of oleic acid-hydroxy stearic acid (OAHSA) and its anti-inflammatory functions are poorly understood. This study investigated the composition of OAHSA isomers in dietary oils and the impact of 12-OAHSA on obesity-induced inflammation. Liquid chromatography with tandem mass spectrometry analysis revealed that various dietary oils, including fish oil, corn oil, palm oil, soybean oil, and olive oil, present a wide variation in OAHSA profiles and amounts. The highest amounts of total OAHSAs are present in olive oil including 12-OAHSA. Compared to vehicle-treated obese mice, administration of 12-OAHSA significantly improved glucose homeostasis, independent of body weight. 12-OAHSA-treated mice displayed significantly reduced accumulation of CD11c+ adipose tissue macrophages, and CD4+/CD8+ adipose tissue T lymphocytes. Concomitantly, the expression of pro-inflammatory cytokine genes and the nuclear factor kappa-light-chain-enhancer of activated B cells signaling pathway were significantly decreased in the 12-OAHSA-treated adipose tissue, while the expression of the anti-inflammatory gene Il10 was markedly increased. Moreover, in vitro cell culture experiments showed that 12-OAHSA significantly inhibited the lipopolysaccharides-induced inflammatory response in macrophages by suppressing the nuclear factor kappa-light-chain-enhancer of activated B cells signaling pathway. Collectively, these results indicated that 12-OAHSA, as a component of olive oil, mitigates obesity-induced insulin resistance by regulating AT inflammation. Therefore, 12-OAHSA could be used as a novel nutritional intervention against obesity-associated metabolic dysregulation.