Determination of relative response factors for chromatographic investigations using NMR spectrometry

In the absence of suitable reference materials for impurity quantitation, laboratories have developed techniques using mass detectors such as the chemical luminescence detector (CLND) and the charged aerosol detector (CAD) to normalize the UV response of each impurity of interest by their molar rati...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2009-07, Vol.49 (5), p.1261-1265
Main Authors: Webster, Gregory K., Marsden, Ian, Pommerening, Cynthia A., Tyrakowski, Christina M., Tobias, Brian
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Drug Contamination
Fundamental and applied biological sciences. Psychology
General pharmacology
Magnetic Resonance Spectroscopy - methods
Medical sciences
Molecular Structure
NMR
Nuclear magnetic resonance spectroscopy
Pesticide Residues - analysis
Pesticide Residues - chemistry
Pharmaceutical Preparations - analysis
Pharmaceutical Preparations - chemistry
Pharmacology. Drug treatments
Reference Standards
Relative response factor
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
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Summary:In the absence of suitable reference materials for impurity quantitation, laboratories have developed techniques using mass detectors such as the chemical luminescence detector (CLND) and the charged aerosol detector (CAD) to normalize the UV response of each impurity of interest by their molar ratios and thus generate relative response factors without requiring isolated and purified compound-specific standards. While effective, these detectors are limited in response and are effective only with specific mobile phase requirements. Nuclear magnetic resonance (NMR) spectrometry has the advantage of allowing the universal detection of protons while not suffering from the limitations observed for CLND, CAD, and other common detectors. The determination of relative response factors using NMR has been successfully applied to several LC methods. An overview of this technique and representative results are presented.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2009.02.027