Protein profile in HBx transfected cells: A comparative iTRAQ-coupled 2D LC-MS/MS analysis

The x protein of HBV (HBx) has been involved in the development of hepatocellular carcinoma (HCC), with a possible link to individual genotypes. Nevertheless, the underlying mechanism remains obscure. In this study, we aim to identify the HBx-induced protein profile in HepG2 cells by LC-MS/MS proteo...

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Bibliographic Details
Published in:Journal of proteomics 2010-06, Vol.73 (8), p.1421-1432
Main Authors: Feng, Huixing, Li, Xi, Niu, Dandan, Chen, Wei Ning
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell migration
Diverse techniques
Fundamental and applied biological sciences. Psychology
HBX
HCC
Hepatitis B virus
iTRAQ-coupled 2-D LC-MS/MS
Molecular and cellular biology
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Summary:The x protein of HBV (HBx) has been involved in the development of hepatocellular carcinoma (HCC), with a possible link to individual genotypes. Nevertheless, the underlying mechanism remains obscure. In this study, we aim to identify the HBx-induced protein profile in HepG2 cells by LC-MS/MS proteomics analysis. Our results indicated that proteins were differentially expressed in HepG2 cells transfected by HBx of various genotypes. Proteins associated with cytoskeleton were found to be either up-regulated (MACF1, HMGB1, Annexin A2) or down-regulated (Lamin A/C). These may in turn result in the decrease of focal adhesion and increase of cell migration in response to HBx. Levels of other cellular proteins with reported impact on the function of extracellular matrix (ECM) proteins and cell migration, including Ca2+-binding proteins (S100A11, S100A6, and S100A4) and proteasome protein (PSMA3), were affected by HBx. The differential protein profile identified in this study was also supported by our functional assay which indicated that cell migration was enhanced by HBx. Our preliminary study provided a new platform to establish a comprehensive cellular protein profile by LC-MS/MS proteomics analysis. Further downstream functional assays, including our reported cell migration assay, should provide new insights in the association between HCC and HBx. LC-MS-MS analysis reveals new cellular signalling elements associated with HBx. [Display omitted]
ISSN:1874-3919
DOI:10.1016/j.jprot.2009.12.004