The cancer cell secretome: A good source for discovering biomarkers?

Cancer is a leading cause of death. Early detection is usually associated with better clinical outcomes. Recent advances in genomics and proteomics raised hopes that new biomarkers for diagnosis, prognosis or monitoring therapeutic response will soon be discovered. Proteins secreted by cancer cells,...

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Bibliographic Details
Published in:Journal of proteomics 2010-09, Vol.73 (10), p.1896-1906
Main Authors: Pavlou, Maria P., Diamandis, Eleftherios P.
Format: Article
Language:English
Subjects:
Cancer biomarkers
Mass spectrometry
Proteomic analysis
Proximal fluids
Secretome
Tissue culture
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Summary:Cancer is a leading cause of death. Early detection is usually associated with better clinical outcomes. Recent advances in genomics and proteomics raised hopes that new biomarkers for diagnosis, prognosis or monitoring therapeutic response will soon be discovered. Proteins secreted by cancer cells, referred also as “the cancer cell secretome”, is a promising source for biomarker discovery. In this review we will summarize recent advances in cancer cell secretome analysis, focusing on the five most fatal cancers (lung, breast, prostate, colorectal, and pancreatic). For each cancer type we will describe the proteomic approaches utilized for the identification of novel biomarkers. Despite progress, identification of markers that are superior to those currently used has proven to be a difficult task and very few, if any, newly discovered biomarker has entered the clinic the last 10 years. (A) Phase contrast (I) and merge FRET-microscopy (II) images of cerebellar granule neurons stained with anti-L-VOCC/Alexa488-IgG and anti-C b 5R/Cy3-IgG. (B) Pixels analysis of neuronal somas: green (empty symbols) and red fluorescence (filled symbols). [Display omitted]
ISSN:1874-3919
DOI:10.1016/j.jprot.2010.04.003