CE–MS for proteomics: Advances in interface development and application

Capillary electrophoresis–mass spectrometry (CE–MS) has emerged as a powerful technique for the analysis of proteins and peptides. Over the past few years, significant progress has been made in the development of novel and more effective interfaces for hyphenating CE to MS. This review provides an o...

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Bibliographic Details
Published in:Journal of proteomics 2012-07, Vol.75 (13), p.3814-3828
Main Authors: Ramautar, Rawi, Heemskerk, Anthonius A.M., Hensbergen, Paul J., Deelder, André M., Busnel, Jean-Marc, Mayboroda, Oleg A.
Format: Article
Language:English
Subjects:
Applications
Biological and medical sciences
Capillary electrophoresis
Diverse techniques
Electrospray ionization
Fundamental and applied biological sciences. Psychology
MALDI
Molecular and cellular biology
Sheath-liquid interface
Sheathless interface
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Summary:Capillary electrophoresis–mass spectrometry (CE–MS) has emerged as a powerful technique for the analysis of proteins and peptides. Over the past few years, significant progress has been made in the development of novel and more effective interfaces for hyphenating CE to MS. This review provides an overview of these new interfacing techniques for coupling CE to MS, covering the scientific literature from January 2007 to December 2011. The potential of these new CE–MS interfacing techniques is demonstrated within the field of (clinical) proteomics, more specifically “bottom-up” proteomics, by showing examples of the analysis of various biological samples. The relevant papers on CE–MS for proteomics are comprehensively summarized in tables, including, e.g. information on sample type and pretreatment, interfacing and MS detection mode. Finally, general conclusions and future perspectives are provided. [Display omitted] ► Recent developments and applications of CE–MS in proteomics are provided. ► Attention paid to emerging technological developments regarding CE–MS interfacing. ► Novel interfacing techniques enable new possibilities for CE–MS in proteomics. ► Applicability of CE–MS illustrated for biological and clinical proteomics studies.
ISSN:1874-3919
DOI:10.1016/j.jprot.2012.04.050