Curcumin prevents proteins expression changes of oxidative phosphorylation, cellular stress response, and lipid metabolism proteins in liver of mice fed a high-fructose diet

• Published in: Journal of proteomics 2022-07, Vol.263, p.104595, Article 104595
• Main Authors: Silva-Gaona, Oscar Gerardo, Hernández-Ortiz, Magdalena, Vargas-Ortiz, Katya, Ramírez-Emiliano, Joel, Garay-Sevilla, Ma. Eugenia, Encarnación-Guevara, Sergio, Pérez-Vázquez, Victoriano
• Format: Article
• Language: English
• Subjects: Curcumin; Diet; Fructose; Liver; Oxidative stress
• ISSN: 1874-3919
• DOI: 10.1016/j.jprot.2022.104595

Increased fructose consumption has been associated with the development of metabolic diseases due to the modification in protein expression, altering metabolic and signaling pathways. Curcumin is a natural compound with a regulatory effect on genes and metabolic pathways. To identify the fructose-induced protein expression changes and the effect of curcumin on the change of protein expression in the liver of mice fed a standard diet and a high fructose diet, to elucidate the global role of curcumin. Four groups (n = 4/group) of male mice (C57BL6J) of six-weeks-old were formed. One group received a standard diet (C); another received curcumin at 0.75% w/w in the feed (C + C); one more received 30% w/v fructose in drinking water (F); and one group received 30% w/v fructose in drinking water and 0.75% w/w curcumin in food (F + C); for 15 weeks. Proteomic analysis was performed by LC-MS/MS, using the label-free technique with the MaxQuant programs for identification and Perseus for expression change analysis. Differentially expressed proteins (fold change ≥1.5 and p