Development of a RP-HPLC method for evaluation of in vitro permeability of voriconazole in the presence of enhancers through rat skin

An isocratic RP-HPLC–UV method for the analysis of voriconazole in skin diffusate samples has been developed and validated. Experimental design was employed to optimize the method. The method was validated as per ICH guidelines. Linearity was observed over the concentration range of 2–100μgmL−1 (r2=...

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Bibliographic Details
Published in:Journal of Saudi Chemical Society 2017-01, Vol.21 (1), p.1-10
Main Authors: Vamsi Krishna, M., Dash, Rajendra N., Venugopal, P., Reddy, B. Jalachandra, Sandeep, P., Madhavi, G.
Format: Article
Language:English
Subjects:
Permeation enhancers
RP-HPLC
Skin diffusate samples
Voriconazole
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Summary:An isocratic RP-HPLC–UV method for the analysis of voriconazole in skin diffusate samples has been developed and validated. Experimental design was employed to optimize the method. The method was validated as per ICH guidelines. Linearity was observed over the concentration range of 2–100μgmL−1 (r2=0.999). Limits of detection and quantification were 0.6 and 2μgmL−1, respectively. Intra-day and inter-day precision (% RSD) was within the ICH limits (⩽2%). The method was successfully used to analyze skin diffusate samples, and the effectiveness of different permeation enhancers was compared with respect to flux and permeability coefficient.
ISSN:1319-6103
DOI:10.1016/j.jscs.2013.07.003