Levels of mRNA for three selenoproteins in skeletal muscle of fetal and newborn pigs

Selenoproteins have wide-ranging functions throughout the body, including important roles in protection against oxidative stress, as well as muscle development and integrity. In this study, prepubertal gilts were randomly assigned to either a Se-sufficient or deficient diet ( n = 12/treatment). Leve...

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Bibliographic Details
Published in:Livestock science 2009-09, Vol.124 (1), p.21-25
Main Authors: Terry, E.N., Michal, J.J., Hostetler, C.E., Kincaid, R.L.
Format: Article
Language:English
Subjects:
animal proteins
complementary DNA
dietary minerals
fetus
gene expression
glutathione peroxidase
Glutathione peroxidase-1
maternal nutrition
messenger RNA
mRNA
neonates
piglets
Pigs
pregnancy
reverse transcriptase polymerase chain reaction
Selenium
Selenoprotein N
Selenoprotein W
selenoproteins
skeletal muscle
swine
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Summary:Selenoproteins have wide-ranging functions throughout the body, including important roles in protection against oxidative stress, as well as muscle development and integrity. In this study, prepubertal gilts were randomly assigned to either a Se-sufficient or deficient diet ( n = 12/treatment). Levels of mRNA coding for the selenoproteins glutathione peroxidase-1 (GPx-1), selenoprotein W (SelW) and selenoprotein N (SelN) and the concentration of Se in fetal and neonatal swine skeletal muscle ( gluteus maximus) were measured at time intervals throughout gestation and immediately after birth. Total RNA was isolated from skeletal muscle, and cDNA was synthesized for real-time RT-PCR analysis of the selected selenoproteins. Selenium concentration in skeletal muscle of fetal and newborn pigs decreased throughout gestation ( P < 0.0001) and was lower ( P < 0.05) when dams were fed the low Se diet. Levels of GPx-1 mRNA in fetal skeletal muscle were unaffected by maternal Se intake nor did GPx-1 mRNA levels change during late gestation. Selenoprotein W mRNA levels increased ( P = 0.01) in fetal skeletal muscle during late gestation but were reduced ( P = 0.05) by low maternal intake of Se. Levels of SelN mRNA showed a gestational time by treatment interaction ( P < 0.05). The increased levels of SelW mRNA in skeletal muscle during late gestation, despite a decrease in Se concentration, indicate that SelW may be an important antioxidant protein to the late term fetus and the newborn.
ISSN:1871-1413
1878-0490
DOI:10.1016/j.livsci.2008.12.005