Bacterial sources and cycling dynamics of amino acids in high and low molecular weight dissolved organic nitrogen in the ocean

• Published in: Marine chemistry 2022-04, Vol.241, p.104104, Article 104104
• Main Authors: Ianiri, Hope L., Shen, Yuan, Broek, Taylor A.B., McCarthy, Matthew D.
• Format: Article
• Language: English
• Subjects: Amino acids; Dissolved organic nitrogen; Microbial degradation; Molecular weight
• ISSN: 0304-4203; 1872-7581
• DOI: 10.1016/j.marchem.2022.104104

Amino acids (AA) represent the most abundant identifiable biomolecule class in marine dissolved organic nitrogen (DON) and provide powerful proxies for DON degradation state. In particular, the D-enantiomers of AA (D-AA) are known to be derived mainly from bacteria, making them ideal tracers for bacterially derived N. However, despite the widespread use of D-AA tracers, it remains unclear if the accumulation of different D-AA species in the ocean indicates that most DON arises from direct bacterial sources or from continual bacterial alteration of eukaryotic algal material. This difference has major implications for our understanding of sources and cycling mechanisms of DON in the ocean. Here, we present the most extensive D-AA suite ever reported in younger, high molecular weight (HMW) DON contrasted with older, low molecular weight (LMW) solid phase extracted (SPE) DON from the central Atlantic and Pacific Oceans. We evaluate D-AA in these two contrasted MW fractions in the context of multiple common AA-based proxies and bulk DOM radiocarbon (Δ14C) data. Specifically, we assess if D-AA in HMW and LMW SPE-DON are most consistent with 1) preformed bacterial source signals, 2) progressive bacterial degradation/alteration of eukaryotic algal sources, or 3) gradual, continued resynthesis/addition of new bacterial biomolecules during ocean circulation. Our results suggest that AA-containing molecules in HMW and LMW SPE-DON fractions are almost entirely distinct, with independent bacterial sources and degradation mechanisms. In HMW DON, all measured indices support a surface-produced, semi-labile component which is progressively altered in the mesopelagic with increasing radiocarbon age. In contrast, for LMW SPE-DON, AA-based proxies showed conflicting results. Some proxies (D/L ratios of most D-AA, non-protein AA, mol% Gly, and %C-AA) indicated LMW SPE-DON was less labile and more degraded than HMW DON. However other proxies (D/L-Ala, the N isotope based ΣV parameter, and the commonly used DI index) indicated equivalent or even less degradation and resynthesis in the isolated LMW material compared to HMW material, suggesting a disconnect in the underlying mechanisms reflected by different proxies. Finally, AA composition and degradation state in the subsurface samples of both HMW and LMW DON varied little with increasing radiocarbon age, suggesting that HMW and LMW pools may cycle independently. Together, our results suggest that AA DON sources, while almost e