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Bimodal gigaporous polystyrene microspheres with glycopolymer surfaces for high-speed protein chromatography
[Display omitted] •The bimodal medium featured with ideal gigapores (2 μm) and mesopores (25 nm).•The bimodal medium has polystyrene skeleton and glycopolymer surface.•The gigapores endowed the medium with convective masstransport for fast separation.•High dynamic adsorption capacity and protein rec...
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Published in: | Microchemical journal 2022-06, Vol.177, p.107273, Article 107273 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | [Display omitted]
•The bimodal medium featured with ideal gigapores (2 μm) and mesopores (25 nm).•The bimodal medium has polystyrene skeleton and glycopolymer surface.•The gigapores endowed the medium with convective masstransport for fast separation.•High dynamic adsorption capacity and protein recovery were achieved on the column.•Three model proteins were baseline separated at 2529 cm/h on the DEAE-HGPS column.
A bimodal gigaporous anion-exchange medium with the ion-exchange capacity of 0.215 mmol/ml was successfully developed based on hydrophilic gigaporous polystyrene (HGPS) microspheres (diameter between 60 and 115 μm). There are two types of pore in the medium, i.e., gigapores (0.3–7 μm) and mesopores (10–60 nm). The presence of gigapores in the medium can induce convective flow of mobile phase within microspheres, therefore contributing to high column permeability (3.55×10−10 m2), high dynamic adsorption capacity and high protein resolution at high flow velocities. The mixture of hemoglobin, transferrin and ovalbumin can be baseline separated within 3 min at 2529 cm/h. All the results indicate that anion-exchange medium as developed is very promising for large scale high-speed protein chromatography. |
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ISSN: | 0026-265X 1095-9149 |
DOI: | 10.1016/j.microc.2022.107273 |