A dual emissive silver-riboflavin complex and nitrogen-doped carbon dot nanoprobe for ratiometric detection of glutathione

• Published in: Microchemical journal 2024-04, Vol.199, p.109996, Article 109996
• Main Authors: Alhazzani, Khalid, Alanazi, Ahmed Z., Mostafa, Aya M., Barker, James, El-Wekil, Mohamed M., Ali, Al-Montaser Bellah H.
• Format: Article
• Language: English
• Subjects: Carbon dots; Dual-emission; Glutathione; Ratiometric; Silver-riboflavin
• ISSN: 0026-265X
• DOI: 10.1016/j.microc.2024.109996

[Display omitted] •Novel dual-emission nanoprobe for selective and sensitive GSH detection.•Utilizes competitive interactions with a silver-riboflavin complex.•Probe composed of mixture of Ag-RF complex (yellow emission) and nitrogen-doped carbon dots (blue emission).•Ratiometric measurement (F525/F440) enables interference-free GSH quantification.•Accurate GSH analysis in spiked human serum samples, promising for real-time monitoring. Glutathione (GSH) is an important antioxidant biomarker with pivotal roles in multiple biological processes. Herein, a novel dual-emission ratiometric fluorescence nanoprobe was developed for selective and sensitive GSH detection by exploiting competitive interactions with a silver-riboflavin (Ag-RF) complex. The nanoprobe consists simply of a mixed solution of Ag-RF complex displaying characteristic yellow emission at 525 nm and nitrogen-doped carbon dots exhibiting blue emission at 440 nm. Due to the strong affinity of GSH toward silver ions, the addition of GSH liberates riboflavin from the Ag-RF complex leading to quenching of adjacent nitrogen-doped carbon dots through fluorescence resonance energy transfer (FRET). By ratiometrically measuring the fluorescence intensities at 525 and 440 nm (F525/F440), GSH levels can be rapidly quantified without interference. This sensor gave excellent linearity (R2 = 0.9986) over 0.05–70 μmol/L GSH with high sensitivity (limit of detection = 0.015 μmol/L). The sensor also provided accurate GSH analysis in spiked human serum samples (96–98.5 % recoveries), validating practical applicability. With advantages of simplicity, selectivity, and low sample volume requirements, this fluorescent nanoprobe holds great promise as a tool for real-time, in-field monitoring of GSH for basic research and clinical investigations.