Cyclohexane-1,2-dione hydrolase: A new tool to degrade alicyclic compounds

Alicyclic alcohols are naturally occurring compounds which can be degraded by microorganisms via cleavage of the ring C–C bond. Denitrifying Azoarcus sp. strain 22Lin grows on cyclohexane-1,2-diol which serves as electron donor and carbon source. The diol is converted to cyclohexane-1,2-dione follow...

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Bibliographic Details
Published in:Journal of molecular catalysis. B, Enzymatic Enzymatic, 2009-11, Vol.61 (1), p.47-49
Main Authors: Fraas, Sonja, Steinbach, Alma K., Tabbert, Anja, Harder, Jens, Ermler, Ulrich, Tittmann, Kai, Meyer, Axel, Kroneck, Peter M.H.
Format: Article
Language:English
Subjects:
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Cyclic alcohols
Cyclohexane-1,2-dione hydrolase
Flavoenzyme
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Thiamine diphosphate
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Summary:Alicyclic alcohols are naturally occurring compounds which can be degraded by microorganisms via cleavage of the ring C–C bond. Denitrifying Azoarcus sp. strain 22Lin grows on cyclohexane-1,2-diol which serves as electron donor and carbon source. The diol is converted to cyclohexane-1,2-dione followed by hydrolysis to the corresponding semialdehyde and oxidation to adipate. The latter two reactions are catalyzed by the thiamine diphosphate-dependent flavoenzyme cyclohexane-1,2-dione hydrolase, the first α-ketolase known so far. Biochemical and structural properties of this new member of the thiamine diphosphate enzyme family will be presented.
ISSN:1381-1177
1873-3158
DOI:10.1016/j.molcatb.2009.03.021