Comparison of the performance of commercial immobilized lipases in the synthesis of different flavor esters

• Published in: Journal of molecular catalysis. B, Enzymatic Enzymatic, 2014-07, Vol.105, p.18-25
• Main Authors: Martins, Andréa B., da Silva, Alexandre M., Schein, Mirela F., Garcia-Galan, Cristina, Záchia Ayub, Marco A., Fernandez-Lafuente, Roberto, Rodrigues, Rafael C.
• Format: Article
• Language: English
• Subjects: Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Enzyme reuse; Esterification; Flavor esters; Fundamental and applied biological sciences. Psychology; Immobilized enzyme; Lipase; Methods. Procedures. Technologies
• ISSN: 1381-1177; 1873-3158
• DOI: 10.1016/j.molcatb.2014.03.021

•Novozym 435, Lipozyme TL-IM and Lipozyme RM-IM were compared to esterification activity.•Their performances were highly dependent on the alcohols and acids used.•Novozym 435 presented, in general, better activity.•Novozym 435 can be reused for 10 batches keeping more than 80% of its initial activity. In this work, it is compared the performance of three commercial lipase preparations (Novozym 435, Lipozyme TL-IM, and Lipozyme RM-IM) in the synthesis of flavor esters obtained by esterification of acetic, propionic, and butyric acids using ethanol, isopropyl alcohol, butanol, or pentanol. A comprehensive comparison was performed verifying activities of these three enzyme preparations versus the different couples of substrates, checking the obtained yields. In general, the longer the acid chain, the higher the reaction yields. Novozym 435 was the most efficient enzyme in most cases, and only Lipozyme RM-IM offered better results than Novozym 435 in the production of ethyl butyrate. Reactions with butyric acid showed the highest conversion rates using all biocatalysts. Using optimal substrates, the reactions catalyzed by the three enzymes were optimized using the response surface methodology, and the catalytic performance of the biocatalysts in repeated batches was assessed. After optimization, yields higher than 90% were obtained for all three enzymes, but Lipozyme TL-IM needed four-times more biocatalyst content than the other two preparations. Novozym 435 kept over 80% of its activity when reused in 9 successive batches, whereas Lipozyme RM-IM can be reused 5 times and Lipozyme TL-IM only 3 times. In general, Novozym 435 showed to be more suitable for these reactions than the other two enzyme preparations.