Spectroscopic interactions of titanium dioxide nanoparticles with pharmacologically active 3(2H)-pyridazinone derivative

• Published in: Journal of molecular liquids 2017-05, Vol.233, p.166-172
• Main Authors: Desai, Vani R., Hunagund, Shirajahammad M., Pujar, Malatesh S., Basanagouda, Mahantesha, Kadadevarmath, Jagadish S., Sidarai, Ashok H.
• Format: Article
• Language: English
• Subjects: 3(2H)-pyridazinone; Benesi–Hildebrand equation; Dynamic quenching; FRET theory; S-V equation; TiO2 NPs
• ISSN: 0167-7322; 1873-3166
• DOI: 10.1016/j.molliq.2017.03.018

Herein, we report the spectroscopic interactions of titanium dioxide (TiO2) nanoparticles (NPs) with pharmacologically active 3(2H)-pyridazinone derivative, viz., 5-(2-Hydroxy-4-methyl-phenyl)-2-phenyl-2H-pyridazin-3-one [HMP] in an ethanol solvent using UV–Visible spectrophotometer, fluorescence spectrophotometer and time-correlated single photon counting technique at room temperature. The observed values of absorption, fluorescence intensity and fluorescence lifetime of HMP molecule decrease with increasing in the TiO2 NPs concentration. From the linear Stern-Volmer (S-V) plot in steady state and transient state which indicates the presence of dynamic quenching. The association constant (ka) and quenching constant (KSV) have been estimated using Benesi–Hildebrand and S-V equations respectively. Further, from the fluorescence data we calculated the binding constant and number of binding sites, the results reveals that there exists one binding site in HMP molecule for TiO2 NPs. In addition, we studied the energy transfer in fluorescence quenching by Forster's non-radiative energy transfer (FRET) theory. Results, signified that the fluorescence quenching of the HMP molecule is due to the energy transfer from HMP molecule to TiO2 NPs. The present investigation may be adopted in solar energy materials and also exploited in a variety of applications such as biological sensing, medical diagnosis etc. [Display omitted] •HMP molecule undergoes fluorescence quenching by TiO2 NPs.•The fluorescence quenching of HMP molecule follows the linear S-V relation.•The nature of fluorescence quenching is purely dynamic.•Energy transfer from HMP molecule to TiO2 NPs.•Investigation is useful for sensitizing the TiO2 NPs for solar energy conversion.