Assessment of the miniaturized liquid Ames microplate format (MPF™) for a selection of the test items from the recommended list of genotoxic and non-genotoxic chemicals

• Published in: Mutation research. Genetic toxicology and environmental mutagenesis 2020-08, Vol.856-857, p.503218, Article 503218
• Main Authors: Spiliotopoulos, Dimitrios, Koelbert, Cécile
• Format: Article
• Language: English
• Subjects: 384-well plate test; Ames MPF test; Ames test; Comparative study; Genotoxicity; Liquid miniaturized microfluidic
• ISSN: 1383-5718; 1879-3592
• DOI: 10.1016/j.mrgentox.2020.503218

•The miniaturized Ames MPF™ was compared to reported Ames tests in Petri dishes.•Recommended chemicals were tested on up to five strains indicated by the OECD.•The comparison of the results shows remarkable concordance between the techniques.•The miniaturized approach is faster, needs less sample and S9, can be automatized. The Ames microplate format (MPF™) is a miniaturized version of the plate agar Ames tests that takes advantage of a liquid microplate approach in 384-well plates with a color change-based readout. This method, already compared to the Ames test in Petri dishes, is used to assess the genotoxic potential of a variety of test items, including (but not limited to) chemicals, environmental samples, and drug candidates. 61 chemicals were selected from the updated recommended lists of genotoxic and non-genotoxic chemicals for assessment of the performance of new or improved genotoxicity tests and tested in up to five bacterial strains. The agreement with the data from the scientific literature (over 90%) confirms the reliability of the Ames MPF™ as a cost-effective and 3R-compliant alternative to the regulatory Ames test that allows to predict and evaluate chemicals’ mutagenicity in a faster, less laborious and, if available, automatable manner.