Chemical constituents from Schisandra perulata and their cytotoxic activity

• Published in: Phytochemistry letters 2021-02, Vol.41, p.65-69
• Main Authors: Anh, Tran Tuan, Doan, Vu Van, Xuan, Vu Thi, Tuan, Bui Quang, Tai, Bui Huu, Kiem, Phan Van, Nhiem, Nguyen Xuan, Cuong, Nguyen The, Park, SeonJu, Seo, Yohan, Namkung, Wan, Kim, Seung Hyun, Mai, Nguyen Thi
• Format: Article
• Language: English
• Subjects: Cytotoxic activity; Dibenzocyclooctadiene; Schisandra perulata; Schisandrulata A; Schisandrulata B
• ISSN: 1874-3900; 1876-7486
• DOI: 10.1016/j.phytol.2020.11.002

[Display omitted] •Two new compounds, schisandrulatas A and B were isolated from Schisandra perulata.•The structures were successfully determined by spectroscopic evidence.•Compounds 1, 4, 7, and 9 showed potent cytotoxic activity against CAL27 cancer cell line.•Compounds 1, 4, 7, and 9 exhibited potent cytotoxic activity against MDA-MB231 cancer cell line. Two new compounds, schisandrulata A (1) and schisandrulata B (5), and nine known compounds, 6-O-benzoylgomisin O (2), kadsutherin (3), γ-schizandrin (4), pinotatol (6), schisphentetralone A (7), (–)-8′-epi-aristotetralone (8), (–)-8,8′-epi-aristotetralone (9), rel-(8R,8′R)-dimethyl-(7S,7′R)-bis(3,4-methylenedioxyphenyl)tetrahydrofuran (10) and (-)-machilusin (11) were isolated from the leaves of Schisandra perulata Gagnep. Their chemical structures were determined by means of HR-ESI-MS, NMR, and CD spectra. All the compounds were evaluated for their cytotoxic activity against the human oral cancer (CAL27) and human breast cancer (MDA-MB231) cell lines. Compounds 1, 4, 7, and 9 showed potent cytotoxic activity against CAL27 cell line with IC50 values of 1.8 ± 0.2, 1.2 ± 0.1, 1.2 ± 0.2, and 2.0 ± 0.1 μM, respectively, indicating that they have stronger cytotoxic activity than that of the positive control capecitabine (IC50 value of 8.20 ± 0.75 μM). Similarly, compounds 1, 4, 7, and 9 also exhibited potent activity against the MDA-MB231 cell line with IC50 values of 3.5 ± 0.1, 1.80 ± 0.2, 0.9 ± 0.2, and 3.4 ± 0.3 μM, respectively. These results indicated that compounds 1, 4, 7, and 9 could be potential anticancer reagents for further drug discovery research.