Phosphate transport by proteoid roots of Hakea sericea

• Published in: Plant science (Limerick) 2007-11, Vol.173 (5), p.550-558
• Main Authors: Sousa, Manuel Fernando, Façanha, Arnoldo Rocha, Tavares, Rui Manuel, Lino-Neto, Teresa, Gerós, Hernâni
• Format: Article
• Language: English
• Subjects: arsenic; Biological and medical sciences; Cell physiology; electrochemistry; Fundamental and applied biological sciences. Psychology; Hakea; Hakea sericea; inorganic phosphorus; Invasive species; kinetics; nutrient uptake; Phosphate uptake; phosphates; Phosphorus-poor soils; physiological transport; Plant physiology and development; Plasma membrane and permeation; Proteaceae; Proteoid roots; protons; root systems; soil nutrients; vanates
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2007.08.006

Up to now the higher capacity of proteoid roots to absorb inorganic phosphate from P i-poor soils has been related mainly to their increased root surface area and higher exudation of organic acids and phosphatases, while much less attention has been directed to their mechanisms of P i uptake. Here we report a characterization of the P i uptake kinetics of the proteoid root-forming species Hakea sericea Schrad. This Proteaceae is an Australian native, which is disseminating very fast through forests of the European south. Dense mats of proteoid roots were observed in the upper soil layers of the invaded area in Portugal where availability of P and N was shown to be very low. Plants grown hydroponically under low-P i also developed proteoid roots, and the proteoid clusters presented a major role in P i absorption in comparison to the non-proteoid portions of the root system as revealed by their higher 32P i labeling. The 32P i uptake by proteoid roots was dependent on H + gradient and yielded a biphasic kinetics, suggesting the involvement of H +/P i co-transport systems with K m values of 0.225 and 40.8 μM P i. The analogs phosphite (Phi) and arsenate, but not vanadate, inhibited competitively the P i absorption. Such biphasic P i uptake pattern with the highest affinity at submicromolar range is likely to be of critical importance for the capacity of this plant species to invade and proliferate throughout vast areas of nutrient-deprived soils.