Biomimetic characterization reveals enhancement of hydroxyapatite formation by fluid flow in gellan gum and bioactive glass composite scaffolds

This study presents production and biomimetic characterization of macroporous composite scaffolds based on gellan gum and nanoparticulate bioactive glass (GG-BAG) under conditions relevant for bone tissue engineering. Formation of hydroxyapatite (HAp) within the scaffolds was investigated in the sim...

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Bibliographic Details
Published in:Polymer testing 2019-07, Vol.76, p.464-472
Main Authors: Zvicer, Jovana, Medic, Ana, Veljovic, Djordje, Jevtic, Sanja, Novak, Sasa, Obradovic, Bojana
Format: Article
Language:English
Subjects:
Biomimetic bioreactor
Biomimetic scaffolds
Bone
Physiological characterization
Tissue engineering
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Summary:This study presents production and biomimetic characterization of macroporous composite scaffolds based on gellan gum and nanoparticulate bioactive glass (GG-BAG) under conditions relevant for bone tissue engineering. Formation of hydroxyapatite (HAp) within the scaffolds was investigated in the simulated body fluid (SBF) during 14 days in two biomimetic bioreactors: perfusion bioreactor (1.1 ml/min SBF flowrate) and a bioreactor with coupled dynamic compression and SBF perfusion (5% strain, 0.68 Hz, 1 h on/1 h off, 1.1 ml/min SBF flowrate). HAp formation was evaluated by scanning electron microscopy (FE-SEM), energy dispersive X-ray (EDS) analysis, Fourier transform infrared spectroscopy (FTIR), and X-ray powder diffraction (XRPD). The superficial SBF velocity of 100 μm/s in perfusion bioreactors induced the formation of abundant cauliflower-like HAp crystals throughout the scaffold interior and flake-like crystals on external surfaces resulting in improved mechanical properties as compared to the initial scaffolds. The obtained results indicate potentials of macroporous GG-BAG scaffolds in conjunction with perfusion bioreactors for bone tissue engineering demonstrating high bioactivity suited for cultures of osteogenic cells. [Display omitted] •Perfusion enhanced hydroxyapatite formation compared to dynamic compression and static conditions.•Cauliflower hydroxyapatite crystals were formed throughout perfused scaffolds.•Repeated dynamic compression induced weakening of the porous polymer network.•Gellan gum and bioactive glass scaffolds have shown high bioactivity in vitro.
ISSN:0142-9418
1873-2348
DOI:10.1016/j.polymertesting.2019.04.004