A Study on VOCs Released by Lung Cancer Cell Line Using GCMS-SPME

• Published in: Procedia chemistry 2016, Vol.20, p.1-7
• Main Authors: Thriumani, Reena, Zakaria, Ammar, Jeffree, Amanina Iymia, Hasyim, Yumi Zuhanis Has-Yun, Helmy, Khaled Mohamed, Omar, Mohammad Iqbal, Shakaff, Ali Yeon, Kamarudin, Latifah Munirah
• Format: Article
• Language: English
• Subjects: gas chromatography mass spectrometry (GCMS); Lung cancer; solid-phase microextraction (SPME); volatile organic compounds (VOCs)
• ISSN: 1876-6196; 1876-6196
• DOI: 10.1016/j.proche.2016.07.027

The gas chromatography mass spectrometry (GCMS) with combination of Solid Phase Micro-extraction (SPME) was used to study the volatile organic compounds (VOCs) which emitted by the in-vitro cultured human cells and compared with documented volatile biomarker of lung cancer. For this purpose, the lung cancer cell (A549) and non-cancerous lung cell (WI38VA13) were cultured in identical growth medium, concurrently. The VOCs in the headspace of the cell cultures and the blank growth media (reference sample) were collected directly from the culture flask using SPME for 15minutes. The results show that two different volatile metabolites were screened out between A549 cells and Wi38VA13 cells. A549 cell found to emit 2 noticeable VOC which are decane and heneicosane. While for WI38VA13, the VOCs released were 1-Heptanol and heptadecane. The acquired VOCs were compared with the previous studies. The findings in this work conclude that the specific VOC of cells can be act as their odour signature and can be used to provide non-invasively screening of lung cancer using gas array sensor devices.