Identification of irradiated refrigerated poultry with the DNA comet assay

• Published in: Radiation physics and chemistry (Oxford, England : 1993) England : 1993), 2004-09, Vol.71 (1), p.189-191
• Main Authors: Villavicencio, A.L.C.H, Araújo, M.M, Marin-Huachaca, N.S, Mancini-Filho, J, Delincée, H
• Format: Article
• Language: English
• Subjects: DNA comet assay; Food irradiation detection; Gamma radiation; Poultry
• ISSN: 0969-806X; 1879-0895
• DOI: 10.1016/j.radphyschem.2004.04.025

Food irradiation could make a significant contribution to the reduction of food-borne diseases caused by harmful bacteria such as Salmonella and parasites. In fact these organisms cause an increasing number of diseases and eventually deaths all over the world, also in industrialized countries. Radiation processing has the advantage that in addition to eliminating pathogens, thereby enhancing food safety, it also extends shelf life through destruction of spoilage organisms. The DNA molecule because of its big size is an easy target for ionizing radiation, therefore, changes in DNA offer potential to be used as a detection method for the irradiation treatment. In our study, poultry has been irradiated and changes in DNA analyzed by the Comet Assay. Samples were packed in plastic bags and irradiated. Doses were 0, 1.5, 3.0 and 4.5 kGy. Immediately after irradiation the samples were returned to the refrigerator (4°C). Samples were analyzed 1 and 10 days after irradiation. This method proved to be an inexpensive and rapid screening technique for qualitative detection of irradiation treatment.