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Embryo quality after sperm selection using density gradient centrifugation or microfluidic technology: a sibling oocyte study

This sibling oocyte study was designed to determine how embryo quality is influenced after sperm selection using microfluidic sperm sorting (MSS) technology compared to a standard density gradient centrifugation (DGC). A single-centre prospective non-inferiority sibling oocyte study was performed be...

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Bibliographic Details
Published in:Reproductive biomedicine online 2024-05, Vol.48, p.104058, Article 104058
Main Authors: Wouters, K., Mateizel, I., Schoemans, C., Segers, I., Van Asbroeck, J., Jankovic, D., Krunic, M., Tournaye, H., De Munck, N.
Format: Article
Language:English
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Summary:This sibling oocyte study was designed to determine how embryo quality is influenced after sperm selection using microfluidic sperm sorting (MSS) technology compared to a standard density gradient centrifugation (DGC). A single-centre prospective non-inferiority sibling oocyte study was performed between October 2023 and February 2024, comparing DGC to MSS. Primary endpoint was embryo utilisation rate. Only ICSI cycles with ejaculated sperm and at least 6 mature oocytes (MII) were included. Sample-size calculation indicated a number of 253 MII for each arm. Each raw semen sample was split in two fractions processed by DGC and by MSS using Zymot 850µl® device. During ICSI, half of the MII were injected with sperm processed by DGC and the other half by MSS. The inseminated oocytes were individually cultured in cleavage/blastocyst medium (Origio) for maximum 7 days. The mean female age was 34.5±4.4 years and partner age was 37.5±5.5 years. Sperm concentration (x106/ml) was similar following DGC (2.9±1.2) or MSS (3.5±5.0) (p=0.16). Progressive motility (% A+B) was significantly lower following DGC vs MSS (74.0%±17.2 vs 89.9%±10.4 (p
ISSN:1472-6483
DOI:10.1016/j.rbmo.2024.104058