A robust high selectivity fluorescence turn-on nanoprobe for peroxynitrite detection in inflammatory cells and mice

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2023-04, Vol.291, p.122381, Article 122381
• Main Authors: Ou, Pinghua, Ran, Hongyan, Ye, Xiaoping, Wang, Junyi, Pang, Meiling, Zhao, Lulu, Chen, Meizi, Li, Xiong, Ma, Yongping, Wang, Ping, Chen, Jin, Luo, Quan, Peng, Yongbo
• Format: Article
• Language: English
• Subjects: Fluorescent nanoprobe; Inflammation; Inflammatory diseases; Peroxynitrite anion (ONOO−)
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2023.122381

[Display omitted] •A robust fluorescence turn-on nanoprobe Rhb-ONOO were constructed for ONOO− detecting.•Rhb-ONOO has excellent detection limits as low as 33 nM.•Rhb-ONOO was successfully applied for ONOO− imaging in live cells and inflammatory mice. Changed levels of intracellular peroxynitrite anion (ONOO−) are closely related to the occurrence and development of inflammation. Specific imaging of ONOO− at sites of inflammation can be of great significance not only for inflammation diagnosis but also for obtaining a deeper understanding of the role of ONOO− in inflammation. Therefore, there is an urgent need for constructing some reliable tools to study the relationship between ONOO− and inflammation in biosystems. In this work, we developed a robust high selectivity fluorescence turn-on nanoprobe (Rhb-ONOO) for inflammation-targeted imaging of ONOO−. The Rhb-ONOO was obtained by self-assembly of amphiphilic Rhb-ONOO, which was constructed by the condensation reaction of the hydrophobic, ONOO−-response and deep red-emitting fluorophore (Rhb) with hydrophilic biopolymer glycol chitosan (GC). Rhb-ONOO showed rapid response towards ONOO− during 60 s, high sensitivity with 19-fold enhancement of fluorescence intensity ratio (I628/I0), and excellent selectivity towards ONOO− over other analytes as well as a good linear relationship was observed between the I628/I0 and the ONOO− concentration range 0–1 μM, with an excellent limit of detection (LOD) of 33 nM. Impressively, it was successfully employed Rhb-ONOO for ONOO− imaging in living inflammatory cells and drug-induced inflammatory mice, illustrating nanoprobe Rhb-ONOO has excellent potential for further study ONOO−-related inflammatory diseases.