Regulation of flower development in Dendrobium crumenatum by changes in carbohydrate contents, water status and cell wall metabolism

• Published in: Scientia horticulturae 2008-12, Vol.119 (1), p.59-66
• Main Authors: Yap, You-Min, Loh, Chiang-Shiong, Ong, Bee-Lian
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; air temperature; beta-galactosidase; beta-mannosidase; Biological and medical sciences; buds; carbohydrate content; Carbohydrates; cell wall components; Cell wall composition; Cell wall hydrolases; cell walls; cellulose; cooling; Dendrobium; dormancy breaking; enzyme activity; Flower development; flowering; flowers; fructans; Fundamental and applied biological sciences. Psychology; hemicellulose; nursery crops; Orchid; ornamental plants; Osmolality; pectinesterase; pectins; plant-water relations; starch; water potential; xylan 1,4-beta-xylosidase
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/j.scienta.2008.06.029

The involvement of carbohydrates, water potential, cell wall components and cell wall-based enzymes in regulating flower development in Dendrobium crumenatum was investigated. Plants were subjected to cold treatment to release floral buds from dormancy, and the various parameters were investigated from young floral bud stage till flower senescence. Development of floral buds was accompanied by progressive decrease in concentrations of fructans and starch. Upon full flower opening, concentration of soluble sugars was maximum, accompanied by a more negative water potential. High pectin methylesterase activity was observed during early bud development and decreased thereafter. Significant increase in activities of β-galactosidase, β-mannosidase and β-xylosidase was also observed during floral bud development. The cell walls of sepals and petals were modified extensively during floral bud and flower development, as observed by changes in the amounts of celluloses, hemicelluloses and total pectin. Pectin solubilisation was also observed to commence during early floral bud development. These results indicated that carbohydrate hydrolysis, osmotic changes and cell wall dissolution that began early in young floral buds, all regulated flower development in this sympodial orchid. Possible applications of the findings in the horticultural industry are discussed.