A system for large scale production of chrysanthemum using microponics with the supplement of silver nanoparticles under light-emitting diodes

• Published in: Scientia horticulturae 2018-02, Vol.232, p.153-161
• Main Authors: Tung, Hoang Thanh, Nam, Nguyen Ba, Huy, Nguyen Phuc, Luan, Vu Quoc, Hien, Vu Thi, Phuong, Truong Thi Bich, Le, Dung Tien, Loc, Nguyen Hoang, Nhut, Duong Tan
• Format: Article
• Language: English
• Subjects: Chrysanthemum; Flowering; LEDs; Microponics; Silver nanoparticle
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/j.scienta.2017.12.063

•Combining micro-propagation and hydroponics (microponics) reduces drawbacks such as being prone to contamination and resource-intensive.•We proposed a microponic system supplemented with 7.5 ppm AgNPs under 70% red LED and 30% blue LED for shoots culture of Chrysanthemum.•The microponic system favored the growth and development as well as effectively reduced microbial counts of the plantlets.•This microponic system is suitable for for commercial production of seedlings. A microponic culture system, combining micro-propagation and hydroponics, could reduce the drawbacks of micro-propagation system such as being prone to contamination, being resource-intensive and require large areas, etc. In this study, chrysanthemum shoots (3 cm in length) were cultured in a microponic system and micro-propagation system. The growth of shoots cultured on half-strength sugar-free liquid MS medium supplemented with 7.5 ppm silver nanoparticles under 70% red LED combined with 30% blue LED were the highest among tested concentrations. Results of qualitative and quantitative tests of microbial contents in the microponic culture by 4 testing methods indicated that at concentration of 7.5 ppm silver nanoparticles effectively reduces microbial counts of 8 tested bacteria (Corynebacterium sp., Enterobacter sp., Arthrobacter sp., Agrobacterium sp., Xanthomonas sp., Pseudomonas sp., Bacillus sp. and Micrococcus sp.) and 3 fungi (Aspergillus sp., Fusarium sp. and Alterneria sp.). After 12 weeks at the nursery stage, the chrysanthemum plants derived from microponics began to bear flower-buds. Chrysanthemums produced by the microponic system started flowering after 15 weeks, which was 1 week earlier than those produced by in vitro micropropagation. Taken together, a microponic system was developed and tested successfully. The shoots derived from microponic culture could flower normally at 1-week earlier than the micropropagation-derived ones. The approach was proved to be both cost and time saving.