N-α-Lauroyl-L-arginine ethyl ester hydrochloride combined with hot water treatment alleviates chilling injury of postharvest cucumber fruit

• Published in: Scientia horticulturae 2023-05, Vol.315, p.111986, Article 111986
• Main Authors: Hou, Yuanyuan, Liu, Lanlan, Liu, Yu, Zhao, Liangyi, Bao, Yinqiu, Zheng, Yonghua, Jin, Peng
• Format: Article
• Language: English
• Subjects: Cold tolerance; Cucumber fruit; LAE+HW treatment; Polyamines; Proline and GABA; ROS homeostasis
• ISSN: 0304-4238
• DOI: 10.1016/j.scienta.2023.111986

•The RSM was applied to optimize the conditions of a combined treatment of LAE and HW.•LAE+HW treatment reduced chilling injury in cucumber fruit during cold storage.•LAE+HW treatment could activate antioxidant enzymes activities to eliminate ROS.•LAE+HW treatment enhanced PAs, proline, and GABA contents in cucumber fruit. Postharvest cucumber fruit is prone to chilling injury (CI) under cold stress, leading to severe quality deterioration. In this study, the effects of N-α-Lauroyl-l-arginine ethyl ester hydrochloride (LAE), hot water (HW), and LAE combined with HW treatments on CI in cucumber fruit stored at 4 ± 1 ℃ were investigated. The response surface methodology (RSM) confirmed that the LAE concentration of 1.00 g L−1, HW temperature of 44 ℃, and immersion time of 10 min were the optimal condition for cucumber fruit preservation during cold storage. Further analysis revealed that LAE, HW, and LAE combined with HW treatments, all significantly mitigated chilling injury symptoms in cucumber fruit in comparison with the control, but the application of LAE combined with HW was the most effective method for enhancing cold tolerance in cucumber fruit. Moreover, LAE combined with HW treatment could reduce reactive oxygen species (ROS) accumulation, and enhance superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX) activities in cold-stored cucumber fruit. Meanwhile, the activities of arginine decarboxylase (ADC), ornithine decarboxylase (ODC), Δ1-pyrroline-5-carboxylate synthetase (P5CS), ornithine δ-aminotransferase (OAT), and glutamate decarboxylase (GAD) were also increased by LAE combined with HW treatment, while the activities of proline dehydrogenase (PDH) and γ-aminobutyric acid transaminase (GABA-T) were decreased by LAE combined with HW treatment, which contributed to maintaining higher polyamines, proline, and GABA contents. These findings suggested that LAE combined with HW treatment can reduce chilling injury in cucumber fruit via enhancing antioxidant enzymes activities, PA, proline, and GABA contents to maintain ROS homeostasis during cold storage.