Altered arsenic availability, uptake, and allocation in rice under elevated temperature

• Published in: The Science of the total environment 2021-04, Vol.763, p.143049, Article 143049
• Main Authors: Farhat, Yasmine A., Kim, Soo-Hyung, Seyfferth, Angelia L., Zhang, Long, Neumann, Rebecca B.
• Format: Article
• Language: English
• Subjects: Arsenic; Arsenic - analysis; Climate change; Food quality; Humans; Oryza; Oryza sativa; Plant Roots - chemistry; Root plaque; Soil; Soil Pollutants - analysis; Temperature
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2020.143049

Climate change is expected to increase growing temperatures in rice cultivating regions worldwide. Recent research demonstrates that elevated temperature can increase arsenic concentrations in rice tissue, exacerbating an existing threat to rice quality and human health. However, the specific temperature-induced changes in the plant-soil system responsible for increased arsenic concentrations remain unclear and such knowledge is necessary to manage human dietary arsenic exposure in a warmer future. To elucidate these changes, we established four temperature treatments in climate-controlled growth chambers and grew rice plants (Oryza sativa cv. M206) in pots filled with Californian paddy soil with arsenic concentrations of 7.7 mg kg−1. The four chosen temperatures mimicked IPCC forecasting for Northern California, with a roughly 2.5 °C increase between treatments (nighttime temperatures ~2 °C cooler). We observed that arsenic concentrations in porewater, root iron plaque, and plant tissue increased in response to elevated temperature. There was a positive linear relationship between temperature and rice grain arsenic, almost all of which was present as inorganic As (III). Above-ground allocation patterns were consistent across treatments. We found no upregulation in the gene encoding the OsABCC1 transporter, believed to be important for arsenic sequestration in vacuoles and thereby preventing arsenic transfer to grain. Rice plants grown at higher temperatures had more adsorbed arsenic per unit of iron plaque (measured as [As]/[Fe]), indicating temperature may impact arsenic sorption to root plaque. We present evidence that increased soil mobilization of arsenic was the driving factor responsible for increased arsenic uptake into rice grain. Transpiration, which can increase arsenic transport to roots, was also heightened with elevated temperature but appeared to play a secondary role. Our system had low soil arsenic concentrations typical for California. Our findings highlight that elevated growing temperatures may increase the risk of dietary arsenic exposure in rice systems that were previously considered low risk. [Display omitted] •Higher temperatures increased arsenic concentrations in all tissues, including grain.•Temperature increased arsenic content but did not alter arsenic allocation patterns.•At higher temperatures root plaques sorbed more arsenic per unit of iron plaque.•Temperature-fueled mobilization of arsenic from soil was likely a key drive