Molecular mechanisms that regulate the heat stress response in sea urchins (Strongylocentrotus intermedius) by comparative heat tolerance performance and whole-transcriptome RNA sequencing

• Published in: The Science of the total environment 2023-11, Vol.901, p.165846, Article 165846
• Main Authors: Han, Lingshu, Hao, Pengfei, Wang, Wenpei, Wu, Yanglei, Ruan, Shuchao, Gao, Chuang, Tian, Wanrong, Tian, Ye, Li, Xiaonan, Wang, Luo, Zhang, Weijie, Wang, Heng, Chang, Yaqing, Ding, Jun
• Format: Article
• Language: English
• Subjects: ceRNA; Heat stress; lncRNA; miRNA; Strongylocentrotus intermedius; Whole-transcriptome RNA-seq
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2023.165846

In the context of climate change and extreme high temperature, the commercially important sea urchin Strongylocentrotus intermedius suffers high mortality during summer in Northern China. How sea urchins respond to high temperatures is of great concern to academia and industry. How to understand the heat tolerance of sea urchin from the whole transcriptome level. In this study, the heat-resistant S. intermedius bred by our team and its control group were used as the research objects, then we applied whole-transcriptome RNA sequencing to detect differentially expressed mRNAs, microRNAs, long noncoding RNAs that respond to heat stress in the heat-resistant and control S. intermedius. A competitive endogenous RNA (ceRNA) regulatory network was constructed with predicted pairs of differentially expressed mRNAs and noncoding RNAs and revealed the molecular regulatory mechanisms in S. intermedius responding to heat stress. A functional analysis suggested that the ceRNAs were involved in basal metabolism, calcium ion transport, endoplasmic reticulum stress, and apoptosis. This is the whole-transcriptomic analysis of S. intermedius under heat stress to propose ceRNA networks that will provide a basis for studying the potential functions of long noncoding RNAs and miRNAs in the heat stress response in S. intermedius and provide a theoretical basis for the study of the molecular mechanism of sea urchins in response to environmental changes. [Display omitted] •The Strongylocentrotus intermedius we bred (phenotype marked R) has a potential to tolerate high temperatures.•The “miRNA-mRNA-lncRNA” network of S. intermedius responding to high temperature was obtained.•Under heat stress, sea urchin activates calcium ion signal transduction and then activates endoplasmic reticulum stress to regulate gene expression.