Engineering a vacuum-actuated peristaltic micropump with novel microchannel design to rapidly separate blood plasma with extremely low hemolysis

A need exists for scalable, automated lab-on-chip systems to separate blood plasma for medical diagnostics. In this study, a vacuum-actuated peristaltic micropump (VPM) was developed, incorporating with the inertial microfluidic technique for the separation and collection of blood plasma from dilute...

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Bibliographic Details
Published in:Sensors and actuators. A. Physical. 2024-12, Vol.379, p.115845, Article 115845
Main Authors: Vo, Tuan Ngoc Anh, Chen, Pin-Chuan, Chen, Pai-Shan, Jair, Yung-Cheng, Wu, Yi-Hsin
Format: Article
Language:English
Subjects:
Inertial microfluidics
Low hemolysis
Micropump
Rapid blood plasma separation
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Summary:A need exists for scalable, automated lab-on-chip systems to separate blood plasma for medical diagnostics. In this study, a vacuum-actuated peristaltic micropump (VPM) was developed, incorporating with the inertial microfluidic technique for the separation and collection of blood plasma from diluted blood. The features of the micropump were investigated by varying parameters such as frequency, vacuum pressure, and the number of microchannels. The highest achievable flow rate was found to be 832 µL/min. Subsequently, to minimize the occurrence of red blood cell rupture during the separation process and significantly reduce hemolysis, the configuration of the vertical wall inside the microchannel was modified to an inclined wall. This improvement was validated through experiments using high-speed cameras and fluorescent particles. Blood plasma separation was achieved with high efficiency (98.5 %), rapidity (
ISSN:0924-4247
DOI:10.1016/j.sna.2024.115845