Differential scanning calorimetry study of early and advanced stages in Parkinson’s disease using human blood serum

• Published in: Thermochimica acta 2018-04, Vol.662, p.64-68
• Main Authors: Michnik, Anna, Sadowska-Krępa, Ewa, Cholewa, Joanna, Schisler, Izabela, Kiełboń, Agnieszka, Drzazga, Zofia
• Format: Article
• Language: English
• Subjects: Differential scanning calorimetry (DSC); Human blood serum; Parkinson’s disease
• ISSN: 0040-6031; 1872-762X
• DOI: 10.1016/j.tca.2018.02.014

•The utility of differential scanning calorimetry in characterizing PD severity.•The dependence of parameters of serum denaturation transition on PD severity.•Blood serum calorimetry does not diagnose the early stage of Parkinson’s disease.•The age contributes to PD-related changes of serum proteins denaturation process.•Oxidative damage to serum proteins may be important in PD progression. The study evaluates the use of differential scanning calorimetry (DSC) in differentiating the severity of Parkinson’s disease (PD). The heat capacity profiles and thermodynamic parameters of serum denaturation transition in advanced PD deviate significantly from the age-matched healthy controls. Unfortunately, blood serum calorimetry does not indicate the early stages of the disease. The amplitude of the main transition and the enthalpy of serum denaturation are significantly reduced with advanced disease. Statistically significant, inverse correlations have been found between these parameters and age as well as disease severity. Significantly higher mean temperature of the second transition at about 70 C, has been observed for PD patients in comparison with healthy participants but only small its deviations have appeared across disease groups. Results of the study indicate that DSC can be used as complementary bioanalytical tool for the monitoring of disease-related changes in serum and PD progression.