Influence of inorganic and organic iron compounds on parameters of cell growth and survival in human colon cells

Increased risk for development of colon cancer is associated with red meat intake and iron toxicity is discussed for one underlying mechanism. Anyhow, for iron itself only limited evidence is found. In this study, effects of different iron compounds on proliferation of HT29 carcinoma and LT97 adenom...

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Bibliographic Details
Published in:Toxicology in vitro 2009-04, Vol.23 (3), p.400-407
Main Authors: Klenow, Stefanie, Pool-Zobel, Beatrice L., Glei, Michael
Format: Article
Language:English
Subjects:
Adult
Cell Proliferation - drug effects
Cell Survival - drug effects
Colon - drug effects
Colon - metabolism
Colon - pathology
Colon cancer
Female
Ferric Compounds - toxicity
Ferrous Compounds - toxicity
Hemin
Hemin - toxicity
Hemoglobin
Hemoglobins - toxicity
HT29 Cells
Humans
Iron
Iron Compounds - toxicity
Nitrilotriacetic Acid - analogs & derivatives
Nitrilotriacetic Acid - toxicity
Proliferation
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Summary:Increased risk for development of colon cancer is associated with red meat intake and iron toxicity is discussed for one underlying mechanism. Anyhow, for iron itself only limited evidence is found. In this study, effects of different iron compounds on proliferation of HT29 carcinoma and LT97 adenoma human colon cells were investigated. After treatment of cells with inorganic (ferrous sulfate: FeSO 4 and ferric nitrilotriacetate: FeNTA) and organic (hemoglobin and hemin) iron sources (24–72 h), number of cells and metabolic activity were measured. Under normal cell culture conditions, neither iron compound elevated cell growth in either cell line with the exception of FeNTA which induced LT97 cell growth significantly. Distinct inhibition of cell proliferation was measured for organic iron. Serum-free incubation of HT29 cells revealed growth promoting properties of iron under deficiency. Even though organic iron, especially hemin, was a potent growth factor, both substances showed also dose-dependent cytotoxic effects. In conclusion, these data emphasize that not iron itself, but merely organic iron may promote carcinogenic events. Since promotion of proliferation was only detectable under deficiency, cytotoxic properties of organic iron may be of more importance in colon carcinogenesis.
ISSN:0887-2333
1879-3177
DOI:10.1016/j.tiv.2009.01.004