Prepupae and pupae transcriptomic characterization of Trichogramma chilonis

• Published in: Genomics (San Diego, Calif.) Calif.), 2020-03, Vol.112 (2), p.1651-1659
• Main Authors: Liu, Jianbai, Yi, Jiequn, Wu, Han, Zheng, Lingyan, Zhang, Guren
• Format: Article
• Language: English
• Subjects: Animals; Gene expression; Gene Expression Regulation, Developmental; Genes, Insect; Hymenoptera - genetics; Hymenoptera - growth & development; Metamorphosis; Pupa - genetics; Pupa - metabolism; Transcriptome; Trichogramma chilonis
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2019.10.003

The egg parasitoid, Trichogramma chilonis, has significant control effects on agriculture and forestry pests and is widely employed in southern China for the biological control of lepidopteran pests. In this study, transcriptomic analysis was used to gain a clear understanding of the molecular changes in prepupae and pupae of T. chilonis. A total of 16.88 Gb of clean data were obtained and finally assembled into 43,136 unigenes, 18,880 of which were annotated. After FPKM standardization, 117 and 838 specific expression genes were found in prepupae and pupae, respectively. There were 3129 differentially expressed genes between prepupae and pupae. Compared to pupae, 806 genes were up-regulated and 2323 were down-regulated in prepupae. Background on the T. chilonis transcriptome, the enriched GO function and KEGG pathway analysis of DEGs were considered. As indicated by GO classification, up-regulated genes were mainly involved in chitin metabolism, cell adhesion and endocytic, while most down-regulated genes were involved in synthesis of cell components, ion transport and biological regulation. KEGG enrichment analysis showed that 458 DEGs were enriched in 94 metabolic pathways. DEGs involved in nucleotide replication and transcription, substance metabolism, insect hormone biosynthesis, cell growth and death, reproductive metabolism, circadian rhythms and signal transduction pathways were up-regulated or down-regulated to different degrees, indicating that these genes played important roles during the process of metamorphosis in T. chilonis. This study provides a rich data source for the future study of T. chilonis molecular and biological mechanisms. A large number of genes related to metamorphosis were found based on comparison analysis between prepupae and pupae transcriptomes. This study lays a good foundation for in-depth study of gene transcription and regulation mechanisms during T. chilonis metamorphosis. •117 and 838 genes were specifically expressed in prepupae and pupae of T. chilonis, respectively.•There were 3,129 differentially expressed genes between prepupae and pupae of T. chilonis.•458 DEGs were enriched in 94 metabolic pathways and played important roles during the process of metamorphosis.•The study helps explore the regulatory mechanisms of gene expression in metamorphosis process of T. chilonis.