Immunosensing of Cardiac Troponin I (cTnI) Using a Two-Electrode Electrochemiluminescence Platform with Near Persisting Luminescence Generated on a Ru(bpy)3 2+-Tripropylamine System

An economical, rapid, and ultrasensitive detection of biomolecules in clinical settings is very crucial, particularly for the early detection of Cardiac Troponin I (cTnI), which is the gold standard biomarker for Acute Myocardial Infarction (AMI). Electrochemiluminescence (ECL) has risen in prominen...

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Bibliographic Details
Published in:ACS applied bio materials 2024-11, Vol.7 (11), p.7700-7709
Main Authors: Kala, Arathy B. K., Rajeevan, Greeshma, Madanan, Anju S., Varghese, Susan, Abraham, Merin K., Shkhair, Ali Ibrahim, Indongo, Geneva, George, Sony
Format: Article
Language:English
Subjects:
Biocompatible Materials - chemistry
Biosensing Techniques
Electrochemical Techniques
Electrodes
Gold - chemistry
Humans
Immunoassay - methods
Luminescent Measurements
Materials Testing
Metal Nanoparticles - chemistry
Particle Size
Propylamines - chemistry
Troponin I - analysis
Troponin I - blood
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Summary:An economical, rapid, and ultrasensitive detection of biomolecules in clinical settings is very crucial, particularly for the early detection of Cardiac Troponin I (cTnI), which is the gold standard biomarker for Acute Myocardial Infarction (AMI). Electrochemiluminescence (ECL) has risen in prominence as an important technique for in vitro diagnosis and detection by virtue of its high sensitivity reaching a femtomolar level. This study introduces an economically feasible nanoplatform for ECL immunosensing, consisting of a gold nanoparticle (AuNP) with Ru­(bpy)3 2+ and tripropylamine (TPA) system, which is a potential ECL luminophore and coreactant system. AuNPs serve the role of an ECL signal enhancer as well as the carrier of antibody, which enables the creation of a label-free immunosensor for antigen–antibody interactions. The prepared immunosensor detected cTnI with a detection limit (LOD) of 0.03 ng/mL. This potential immunosensor provides appreciable results in the detection of cTnI from spiked real serum analysis, which shows its potential application in low-resource clinical settings.
ISSN:2576-6422
2576-6422
DOI:10.1021/acsabm.4c01247