Stabilizing Effect of a Multiple Salt Bridge in a Prenucleated Peptide

• Published in: Journal of the American Chemical Society 1998-10, Vol.120 (41), p.10643-10645
• Main Authors: Mayne, Leland, Englander, S. Walter, Qiu, Rong, Yang, Jianxin, Gong, Youxiang, Spek, Erik J, Kallenbach, Neville R
• Format: Article
• Language: English
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/ja981592c

The free energy contribution of salt bridges to protein stability remains a controversial issue. The role of single salt bridges has been investigated in protein and peptide studies; there is no reported value of a multiple salt bridge employing peptide models. We have designed a de novo peptide model to evaluate the strength of a multiple salt bridge. Measurement of the rate of hydrogen exchange (HX) of the backbone NH groups in α-helical peptides allows determination of the absolute free energy of individual interactions that participate in helix stabilization. We apply this procedure here to evaluate the stabilizing contribution of a multiple salt bridge involving Glu3, Asp4, and Arg7 in an 11-mer peptide. The peptide is prenucleated by a lactam bridge connecting two side chains spaced four residues apart. Measurement of the HX rates of each NH group in this peptide as a function of pH, together with the pH independent rate of a Gln3 and Asn4 analogue, reveals a substantial favorable contribution to the α-helix from the salt bridge:  ΔG° = −1.2 ± 0.2 kcal/mol. This is the first evaluation of the strength of an engineered complex salt bridge in a peptide and yields a high value, comparable to results of mutation studies on proteins.