Preparation of Microcapsulated Enzymes for Lowering the Allergenic Activity of Foods

Alcalase from Bacillus licheniformis was microcapsulated by the spray-dry method for degradation of ovalbumin (OVA) in the small intestine. Capsules were prepared with yield of about 65% and a mean particle diameter of 10−20 μm. The microcapsulated alcalase was very resistant to digestion in the gas...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1997-10, Vol.45 (10), p.4178-4182
Main Authors: Yoshimaru, Tetsuro, Matsumoto, Kiyoshi, Kuramoto, Yuichiro, Yamada, Koji, Sugano, Michihiro
Format: Article
Language:English
Subjects:
ABSORCION DIGESTIVA
ABSORPTION DIGESTIVE
ALBUMINAS
ALBUMINE
ALBUMINS
ALERGENOS
ALERGIA ALIMENTARIA
ALLERGENE
ALLERGENS
ALLERGIE ALIMENTAIRE
Animal, plant, fungal and microbial proteins, edible seaweeds and food yeasts
BACILLUS LICHENIFORMIS
Biological and medical sciences
Biotechnology
DIGESTIVE ABSORPTION
FOOD ALLERGIES
Food industries
Fundamental and applied biological sciences. Psychology
Immobilization of enzymes and other molecules
Immobilization techniques
INTESTINAL ABSORPTION
Methods. Procedures. Technologies
MICROENCAPSULACION
MICROENCAPSULATION
OVALBUMIN
PROTEASAS
PROTEASE
PROTEASES
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Summary:Alcalase from Bacillus licheniformis was microcapsulated by the spray-dry method for degradation of ovalbumin (OVA) in the small intestine. Capsules were prepared with yield of about 65% and a mean particle diameter of 10−20 μm. The microcapsulated alcalase was very resistant to digestion in the gastric environment (pH 1.2), and the efficiency of release was about 100% within 10 min in intestinal fluids (pH 6.8). Moreover, when microcapsules were incubated with a solution of OVA, >99% of OVA was hydrolyzed within 120 min. In addition, microcapsules of protease YP-SS from Aspergillus niger were also prepared for degradation of OVA in the stomach. Almost all protease YP-SS activity was released from capsules within only 10 min in gastric fluids. Oral administration of the microcapsulated enzyme to Sprague-Dawley rats resulted in a significant decrease in the serum level of OVA-specific IgE. Keywords: Microcapsulated enzymes; degradation of OVA; Eudragit; spray-dry method; oral administration test
ISSN:0021-8561
1520-5118
DOI:10.1021/jf970379h