Analysis of Free and Esterified Ergosterol in Tomato Products

A new extraction and chromatographic procedure to quantify free and esterified ergosterol in tomato products was devised. The extraction solution was composed of a dichloromethane/methanol mixture in a 2:1 (v/v) ratio. This extraction solvent allowed for higher ergosterol recovery from tomato produc...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2000-03, Vol.48 (3), p.780-784
Main Authors: de Sio, Francesco, Laratta, Bruna, Giovane, Alfonso, Quagliuolo, Lucio, Castaldo, Domenico, Servillo, Luigi
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromatography, High Pressure Liquid
Ergosterol - analysis
Ergosterol - chemistry
Esters
Food industries
Food Microbiology
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
General aspects
Humans
Lycopersicon esculentum - chemistry
Lycopersicon esculentum - microbiology
Methods of analysis, processing and quality control, regulation, standards
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Summary:A new extraction and chromatographic procedure to quantify free and esterified ergosterol in tomato products was devised. The extraction solution was composed of a dichloromethane/methanol mixture in a 2:1 (v/v) ratio. This extraction solvent allowed for higher ergosterol recovery from tomato products (an average of 25% more) compared to hexane, which is frequently employed for ergosterol extraction. Both free and esterified ergosterol were determined by HPLC reverse-phase chromatography employing a Nova-Pak C-18 column (300 × 3.9 mm), filled with 4 mm average particle size and a guard column of the same material. The elution was performed at a flow rate of 1 mL·min-1 with a linear gradient of solvent A (methanol/water, 80:20, v/v) and solvent B (dichloromethane). The gradient, starting at sample injection, was from 0 to 50% B for 20 min for the free ergosterol analysis and additional 15 min at 50% B to analyze the ergosterol esters. This technique has proven to be more sensitive for ergosterol determination than other reported chromatographic procedures. Moreover, ergosterol esters, extracted from various fungal sources, separated well and were easily quantified. Keywords: Ergosterol; tomato; fungal contamination; microbiological quality
ISSN:0021-8561
1520-5118
DOI:10.1021/jf990475d