Persistence Length of Single-Stranded DNA

The self-diffusion coefficient of a series of DNA fragments ranging from 280 to 5386 bases has been measured by fluorescence recovery after photobleaching after thermal denaturation in 8 M urea. The total persistence length p of single-stranded DNAs and its variation in ionic strength down to 10-3 M...

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Bibliographic Details
Published in:Macromolecules 1997-09, Vol.30 (19), p.5763-5765
Main Authors: Tinland, Bernard, Pluen, Alain, Sturm, Jean, Weill, Gilbert
Format: Article
Language:English
Subjects:
Applied sciences
Biological and medical sciences
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
In solution. Condensed state. Thin layers
Miscellaneous
Molecular biophysics
Natural polymers
Physico-chemical properties of biomolecules
Physicochemistry of polymers
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Summary:The self-diffusion coefficient of a series of DNA fragments ranging from 280 to 5386 bases has been measured by fluorescence recovery after photobleaching after thermal denaturation in 8 M urea. The total persistence length p of single-stranded DNAs and its variation in ionic strength down to 10-3 M has been deduced. The importance of the value of p versus the pore size a and contour length L of the DNA in the optimization of sequencing by gel electrophoresis is emphasized.
ISSN:0024-9297
1520-5835
DOI:10.1021/ma970381+