Selectable marker genes engineered for specific expression in target cells for plant transformation

A wound-induced promoter (AoPRl) isolated from Asparagus officinalis was shown by GUS reporter gene analysis to be active during callus formation in tissue cultured leaves from transgenic tobacco plants. Unlike other promoters commonly used to drive expression of marker genes during transformation (...

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Bibliographic Details
Published in:Bio/Technology 1993-02, Vol.11 (2), p.218-221
Main Authors: Ozcan, S. (University of Leicester, Leicester, UK), Firek, S, Draper, J
Format: Article
Language:English
Subjects:
Agriculture
AGROBACTERIUM TUMEFACIENS
ASPARAGUS OFFICINALIS
BETA-GLUCURONIDASE
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
CAL
CALLO
CALLUS
EXPRESION GENICA
EXPRESSION DES GENES
FEUILLE
FOSFOTRASFERASA
Fundamental and applied biological sciences. Psychology
GENE
GENE EXPRESSION
GENES
Genetic engineering
Genetic technics
GENETIC TRANSFORMATION
GLICOSIDASAS
GLYCOSIDASE
GLYCOSIDASES
HOJAS
LEAVES
Life Sciences
Methods. Procedures. Technologies
neomycin phosphotransferase ii
NICOTIANA TABACUM
PHOSPHOTRANSFERASE
PHOSPHOTRANSFERASES
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
PROMOTERS
RACINE
RAICES
REPORTER GENES
ROOTS
SOLANUM TUBEROSUM
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
Transgenic animals and transgenic plants
TRANSGENIC PLANTS
TUBERCULE
TUBERCULO
TUBERS
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Description
Summary:A wound-induced promoter (AoPRl) isolated from Asparagus officinalis was shown by GUS reporter gene analysis to be active during callus formation in tissue cultured leaves from transgenic tobacco plants. Unlike other promoters commonly used to drive expression of marker genes during transformation (e.g. Nos, MAS-35S and CaMV35S), the AoPR1 promoter showed strong expression at wound sites during tobacco leaf disk transformation but was expressed at extremely low levels in the leaves, roots and seeds of the mature plant. A plant transformation vector was constructed in which nptII expression was placed under the control of the AoPR1 promoter. This construct was then used in transformation experiments which resulted in the production of a large number of transgenic tobacco and potato plants. In leaves, roots and tubers (in the case of potato) of these plants, the marker gene protein product (NPTII) was present in low amounts compared to the levels found in control transgenic plants produced using a CaMV35S- nptII gene as a selectable marker.
ISSN:0733-222X
1087-0156
2331-3684
1546-1696
DOI:10.1038/nbt0293-218