Rapid virus-free production of recombinant yellow fever virus envelope protein and its in-depth biophysical analysis

The aim of this study was to develop a rapid and easy-to-adapt protocol for recombinant production of Orthoflavivirus proteins. We introduced the new-generation pOpiE2 vector for virus-free transient transfection of Trichopluisa ni High Five insect cells to produce the envelope protein ectodomain of...

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Bibliographic Details
Published in:Scientific reports 2025-12, Vol.15 (1), p.45666-11, Article 45666
Main Authors: Schelle, Luca, Kuttenlochner, Wolfgang, Sanchez, Victoria, Steigenberger, Barbara, Finkl, Katja, Schmid, Marius, Douat, Celine, Halldorsson, Einar, Schubert, Maren, Suppmann, Sabine
Format: Article
Language:English
Subjects:
631/1647
631/326
631/337
631/45
631/61
Antibodies
Dengue fever
Encephalitis
Humanities and Social Sciences
Insect cells
Mass spectrometry
Mass spectroscopy
multidisciplinary
Peptides
Proteins
Quality control
Science
Science (multidisciplinary)
Transfection
Vaccines
Vector-borne diseases
Viral envelope proteins
Viruses
West Nile virus
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