A poloxamer-polypeptide thermosensitive hydrogel as a cell scaffold and sustained release depot

• Published in: Polymer chemistry 2016-01, Vol.7 (17), p.2976-2985
• Main Authors: Lin, Ji-Yu, Lai, Po-Liang, Lin, Yuan-Kai, Peng, Sydney, Lee, Li-Yu, Chen, Chieh-Nan, Chu, I-Ming
• Format: Article
• Language: English
• Subjects: Charge; Charging; Copolymers; Drug delivery systems; Hydrogels; Proteins; Scaffolds; Thermosensitive
• ISSN: 1759-9954; 1759-9962
• DOI: 10.1039/c5py02067k

Herein, we report the synthesis and characterization of a novel positively charged thermosensitive hydrogel prepared from poloxamer (PLX)-poly( l -alanine-lysine) (Lys-Ala-PLX-Ala-Lys) that demonstrates potential in biomedical applications including tissue engineering and drug delivery. At a dilute concentration, the copolymer self-assembled into micelles that were sensitive to temperature elevation. In the concentration range of 3-7 wt%, the aqueous copolymer solution underwent sol-gel transition near the physiological temperature and exhibited changes in its secondary structure content, as evidenced by FT-IR. Excellent viability of cells cultured within the scaffold was observed after 72 h of incubation. On the other hand, negatively charged bovine serum albumin was incorporated into the hydrogel without diminishing material integrity. Over the course of seven days, the protein underwent extended zero-order release. These findings highlight the potential of this system as a cell scaffold as well as a depot for the extended delivery of drugs and proteins, specifically those which bear a charge opposite to that of the hydrogel. Herein, we report the synthesis and characterization of a novel positively charged thermosensitive hydrogel prepared from poloxamer (PLX)-poly( l -alanine-lysine) (Lys-Ala-PLX-Ala-Lys) that demonstrates potential in biomedical applications including tissue engineering and drug delivery.