High spatial and temporal resolution cell manipulation techniques in microchannels

The advent of microfluidics has enabled thorough control of cell manipulation experiments in so called lab on chips. Lab on chips foster the integration of actuation and detection systems, and require minute sample and reagent amounts. Typically employed microfluidic structures have similar dimensio...

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Bibliographic Details
Published in:Analyst (London) 2016-03, Vol.141 (6), p.1888-195
Main Authors: Novo, Pedro, Dell'Aica, Margherita, Janasek, Dirk, Zahedi, René P
Format: Article
Language:English
Subjects:
Actuators
Animals
Chips
Cytological Techniques - instrumentation
Cytological Techniques - methods
Design engineering
Humans
Microchannels
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Microfluidics
Quenching
Reagents
Spatio-Temporal Analysis
Strategy
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Summary:The advent of microfluidics has enabled thorough control of cell manipulation experiments in so called lab on chips. Lab on chips foster the integration of actuation and detection systems, and require minute sample and reagent amounts. Typically employed microfluidic structures have similar dimensions as cells, enabling precise spatial and temporal control of individual cells and their local environments. Several strategies for high spatio-temporal control of cells in microfluidics have been reported in recent years, namely methods relying on careful design of the microfluidic structures ( e.g. pinched flow), by integration of actuators ( e.g. electrodes or magnets for dielectro-, acousto- and magneto-phoresis), or integrations thereof. This review presents the recent developments of cell experiments in microfluidics divided into two parts: an introduction to spatial control of cells in microchannels followed by special emphasis in the high temporal control of cell-stimulus reaction and quenching. In the end, the present state of the art is discussed in line with future perspectives and challenges for translating these devices into routine applications. Reviewing latest developments on lab on chips for enhanced control of cells' experiments.
ISSN:0003-2654
1364-5528
DOI:10.1039/c6an00027d