Identification of the binding site between bovine serum albumin and ultrasmall SiC fluorescent biomarkers

Ultrasmall silicon carbide nanoparticles (SiC USNPs) are very promising biomarkers for developing new applications in diagnostics, cell monitoring or drug delivery, even though their interaction with biological molecules such as different proteins has not yet been investigated in detail. In this stu...

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Bibliographic Details
Published in:Physical chemistry chemical physics : PCCP 2018, Vol.2 (19), p.13419-13429
Main Authors: Dravecz, Gabriella, Jánosi, Tibor Z, Beke, Dávid, Major, Dániel Á, Károlyházy, Gyula, Erostyák, János, Kamarás, Katalin, Gali, Ádám
Format: Article
Language:English
Subjects:
Animals
Binding Sites
Biomarkers
Biomarkers - chemistry
Carbon Compounds, Inorganic - chemistry
Cattle
Dependence
Drug delivery systems
Fluorescence
Fluorescence Resonance Energy Transfer
Fluorescent Dyes - chemistry
Kinetics
Nanoparticles
Nanoparticles - chemistry
Particle Size
Protein Binding
Proteins
Serum albumin
Serum Albumin, Bovine - chemistry
Silicon carbide
Silicon Compounds - chemistry
Spectrometry, Fluorescence
Spectroscopy, Fourier Transform Infrared - methods
Surface Properties
Thermodynamics
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Summary:Ultrasmall silicon carbide nanoparticles (SiC USNPs) are very promising biomarkers for developing new applications in diagnostics, cell monitoring or drug delivery, even though their interaction with biological molecules such as different proteins has not yet been investigated in detail. In this study, the biological behaviour of SiC USNPs in a medium modeling a living organism was investigated in detail through the dependence of the fluorescence on interactions between bovine serum albumin (BSA) and SiC USNPs. The interaction shows transient nanoparticle-protein associations due to the restricted diffusion behaviour of the nanoparticles in the vicinity of a protein. The transient association manifests in a complex fluorescence quenching mechanism where the dynamic component was dominated by Förster resonance energy transfer. By studying SiC nanoparticles of different sizes, it can be concluded that the transient effect is an ultrasmall nanoparticle behaviour. Combined experimental and theoretical studies propose a delayed diffusion model for describing the interaction between ultrasmall NPs and proteins.
ISSN:1463-9076
1463-9084
DOI:10.1039/c8cp02144a