Dietary oleacein, a secoiridoid from extra virgin olive oil, prevents collagen-induced arthritis in mice

• Published in: Food & function 2024-01, Vol.15 (2), p.838-852
• Main Authors: Rosillo, María Ángeles, Villegas, Isabel, Vázquez-Román, Victoria, Fernández-Santos, José María, Ortega-Vidal, Juan, Salido, Sofía, González-Rodríguez, María Luisa, Alarcón-de-la-Lastra, Catalina
• Format: Article
• Language: English
• Subjects: Animal models; Animals; Arthritis; Collagen; Diet; Functional foods & nutraceuticals; GA-binding protein; Gelatinase B; Immunization; Inflammation; Interleukins; Joints (anatomy); Matrix metalloproteinase; Matrix metalloproteinases; Metalloproteinase; NF-κB protein; Nitric-oxide synthase; Olive oil; Prostaglandin E2; Prostaglandin-E synthase; Rheumatoid arthritis; Signal transduction; Tumor necrosis factor-α
• ISSN: 2042-6496; 2042-650X
• DOI: 10.1039/d3fo04240e

Olacein (OLA), one of the main secoiridoids derived from extra virgin olive oil (EVOO), has been shown to modulate oxidative and inflammatory responses in various pathological conditions; however, its potential benefit in joint disorders such as rheumatoid arthritis (RA) is unknown. Therefore, this study was designed to evaluate the preventive role of the effects of an OLA-supplemented diet in the murine model of collagen-induced arthritis (CIA), delving into the possible mechanisms and signaling pathways involved. Animals were fed an OLA-enriched preventive diet for 6 weeks prior to CIA induction and until the end of the experimental time course. On day 43 after the first immunization, mice were sacrificed: blood was collected, and paws were histologically and biochemically processed. Dietary OLA prevented collagen-induced rheumatic bone, joint and cartilage conditions. Circulating matrix metalloproteinase (MMP)-3 and proinflammatory cytokine (IL-6, IL-1β, TNF-α, IL-17) levels were significantly decreased in the joint, as well as MMP-9 and cathepsin-K (CatK) expression in secoiridoid-fed animals. In addition, dietary OLA was able to decrease COX-2, mPGES-1 and iNOS protein expressions and, also, PGE 2 levels. The mechanisms possibly involved in these protective effects could be related to the activation of the Nrf-2/HO-1 axis and the inhibition of proinflammatory signaling pathways, including JAK-STAT, MAPKs and NF-κB, involved in the production of inflammatory and oxidative mediators. These results support the interest of OLA, as a nutraceutical intervention, in the management of RA. Nutritional therapy has been considered a promising approach in RA management. OLA might provide a new dietary strategy in immunoinflammatory-mediated diseases.