Artificial Ribozyme-Based Regulators of Gene Expression

Abstract The development of RNA-based switches of gene expression is summarized. Most switches are based on the SCHISTOSOMA MANSONII hammerhead ribozyme (HHR), a self-cleaving RNA sequence that is inserted into an mRNA. Control of HHR self-cleavage is achieved by the attachment of an aptamer domain...

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Bibliographic Details
Published in:Synlett 2011-07, Vol.2011 (11), p.1486-1494
Main Authors: Seemann, Isabelle T., Hartig, Jörg S.
Format: Article
Language:English
Subjects:
account
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Nucleic acids
Rna, ribonucleoproteins
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Summary:Abstract The development of RNA-based switches of gene expression is summarized. Most switches are based on the SCHISTOSOMA MANSONII hammerhead ribozyme (HHR), a self-cleaving RNA sequence that is inserted into an mRNA. Control of HHR self-cleavage is achieved by the attachment of an aptamer domain to the HHR scaffold. External addition of a small-molecule ligand regulates catalytic activity of the ribozyme and hence gene expression. These so-called aptazymes are suited to control several classes of RNA. In addition to mRNA, we have incorporated artificial RNA switches into tRNA as well as the bacterial 16S rRNA. In addition, the presented switches should be widely applicable as demonstrated by experiments in bacteria, yeast, and mammals. 1 Introduction 2 Ribozymes in Nature 3 Ribozymes as Parts for Artificial mRNA Switches 4 Regulation of Further RNA Classes 5 Conclusion
ISSN:0936-5214
1437-2096
DOI:10.1055/s-0030-1260583