Liquid filling in standard circular well microplates

It is often assumed that droplets dispensed into standard microplate wells will automatically fill their bottoms. We show here by computational simulation and experimental verification that the ability to fill the well bottom is dependent on the surface wetting characteristics. The release of drople...

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Bibliographic Details
Published in:Journal of applied physics 2010-12, Vol.108 (12), p.124701-124701-6
Main Authors: Lu, Gabriel, Tan, Han Yen, Neild, Adrian, Liew, Oi Wah, Yu, Yang, Ng, Tuck Wah
Format: Article
Language:English
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Summary:It is often assumed that droplets dispensed into standard microplate wells will automatically fill their bottoms. We show here by computational simulation and experimental verification that the ability to fill the well bottom is dependent on the surface wetting characteristics. The release of droplets at the center was also found to fill the well bottom better than droplet dispensation in contact with the well wall. Hydrophobic surfaces required higher liquid volumes to fill the well bottom; unlike the case with capillary wells microplates. This renders standard microplate wells less amenable for use in small volume liquid handling; a feature that is increasingly sought after in screening and studies involving scarce agents.
ISSN:0021-8979
1089-7550
DOI:10.1063/1.3525097