Stimulation of PrPC Retrograde Transport toward the Endoplasmic Reticulum Increases Accumulation of PrPSc in Prion-infected Cells

Prion diseases are fatal and transmissible neurodegenerative disorders characterized by the accumulation of an abnormally folded isoform of the cellular prion protein (PrPC) denoted PrPSc. To identify intracellular organelles involved in PrPSc formation, we studied the role of the Ras-related GTP-bi...

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Bibliographic Details
Published in:The Journal of biological chemistry 2002-10, Vol.277 (41), p.38972-38977
Main Authors: Béranger, Florence, Mangé, Alain, Goud, Bruno, Lehmann, Sylvain
Format: Article
Language:English
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Summary:Prion diseases are fatal and transmissible neurodegenerative disorders characterized by the accumulation of an abnormally folded isoform of the cellular prion protein (PrPC) denoted PrPSc. To identify intracellular organelles involved in PrPSc formation, we studied the role of the Ras-related GTP-binding proteins Rab4 and Rab6a in intracellular trafficking of the prion protein and production of PrPSc. When a dominant-negative Rab4 mutant or a constitutively active GTP-bound Rab6a protein was overexpressed in prion-infected neuroblastoma N2a cells, there was a marked increase of PrPSc formation. By immunofluorescence and cell fractionation studies, we have shown that expression of Rab6a-GTP delocalizes PrP within intracellular compartments, leading to an accumulation in the endoplasmic reticulum. These results suggest that prion protein can be subjected to retrograde transport toward the endoplasmic reticulum and that this compartment may play a significant role in PrPSc conversion.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M205110200