Ultrastructural changes in hepatic sinusoidal endothelial cells acutely exposed to colloidal iron

Hepatic sinusoidal endothelial cells form an important interface between the vascular system, represented by the sinusoids, and the space of Disse that surrounds the hepatocyte microvilli. This study aimed to assess the light microscopic and ultrastructural effects of acute exposure of hepatic sinus...

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Bibliographic Details
Published in:Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2003, Vol.55 (1), p.11-16
Main Authors: Bassett, Mark L., Dahlstrom, Jane E., Taylor, Matthew C., Koina, Mark E., Maxwell, Lesley, Francis, Douglas, Jain, Sanjiv, McLean, Allan J.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
colloid
Colloids
Electron Probe Microanalysis
endothelial
Female
Hepatic
Injections, Intravenous
iron
Iron - analysis
Iron Compounds - administration & dosage
Iron Compounds - toxicity
Kupffer Cells - drug effects
Kupffer Cells - ultrastructure
Male
Maltose
Medical sciences
Microscopy, Electron
Microvilli - drug effects
Microvilli - ultrastructure
Rats
Rats, Wistar
sinusoidal
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Summary:Hepatic sinusoidal endothelial cells form an important interface between the vascular system, represented by the sinusoids, and the space of Disse that surrounds the hepatocyte microvilli. This study aimed to assess the light microscopic and ultrastructural effects of acute exposure of hepatic sinusoidal endothelial cells to colloidal iron by injection of rats with iron polymaltose. Eight minutes after a single intravenous injection of iron polymaltose sinusoidal endothelial cells showed defenestration, and thickening and layering as assessed by transmission electron microscopy. Kupffer cells and stellate cells appeared activated. These changes were not observed in control animals, experiments using equivalent doses of maltose, or experiments using colloidal carbon except for Kupffer cell activation due to colloidal carbon. No significant light microscopic changes were seen in study or control animals. The findings indicate that acute exposure to colloidal iron causes changes in hepatic sinusoidal endothelial cells, stellate cells and Kupffer cells. This may be the result of a direct toxic effect of iron or increased production of reactive oxygen species. These observations suggest a possible mechanism for defenestration of sinusoidal endothelial cells in ageing and in disease states.
ISSN:0940-2993
1618-1433
DOI:10.1078/0940-2993-00296