Rapid and Sensitive Analysis of Diclofenac in Human Plasma by GC/SIM/MS

An analytical method for the determination of diclofenac with tolfenamic acid as the internal standard was developed and validated in human plasma by capillary gas chromatography-mass spectrometry (GC/MS). After the addition of the internal standard, the compounds were extracted from plasma at acidi...

Full description

Saved in:
Bibliographic Details
Published in:Analytical letters 1999-01, Vol.32 (11), p.2245-2253
Main Authors: Choi, Man Ho, Choi, Young Kweon, Chung, Bong Chul
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Diclofenac
GC/MS
General pharmacology
Medical sciences
NSAIDs
Pentafluoropropionic anhydride
Pharmacology. Drug treatments
Trimethylsilyation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An analytical method for the determination of diclofenac with tolfenamic acid as the internal standard was developed and validated in human plasma by capillary gas chromatography-mass spectrometry (GC/MS). After the addition of the internal standard, the compounds were extracted from plasma at acidic pH into diethylether, which was then evaporated to dryness. The compounds were derivatized with pentafluoropropionic anhydride (PFPA) and a mixture (1000:2:3, v/w/w) of N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA), ammonium iodide (NH 4 I), and dithioerythritol (DTE). They were determined by GC/MS at m/z 349 (a molecular ion) for diclofenac and m/z 270 (a base ion) for tolfenamic acid. The recovery of this procedure was 97.8%, and the linearity for calibration was 0.9907 as the coefficient factor. The detection and quantitation limits were 0.1 and 0.5 ng/mL, respectively.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032719908542967