A Laboratory Exercise for Genotyping Two Human Single Nucleotide Polymorphisms

The dramatic decrease in the cost of sequencing a human genome is leading to an era in which a wide range of students will benefit from having an understanding of human genetic variation. Since over 90% of sequence variation between humans is in the form of single nucleotide polymorphisms (SNPs), a...

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Bibliographic Details
Published in:Journal of biological education 2016-01, Vol.50 (1), p.73-85
Main Authors: Fernando, James, Carlson, Bradley, LeBard, Timothy, McCarthy, Michael, Umali, Finianne, Ashton, Bryce, Rose, Ferrill F.
Format: Article
Language:English
Subjects:
Bitter taste
College Science
College Students
DNA sequencing
Genetic variation
Genetics
Genomics
Laboratory Experiments
Molecular Biology
Nebraska
Science Activities
Science Experiments
Science Instruction
Science Laboratories
Science Tests
Single nucleotide polymorphism
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Summary:The dramatic decrease in the cost of sequencing a human genome is leading to an era in which a wide range of students will benefit from having an understanding of human genetic variation. Since over 90% of sequence variation between humans is in the form of single nucleotide polymorphisms (SNPs), a laboratory exercise has been devised in order to illustrate the importance of SNPs. Two separate SNPs are analysed, one of which has a significant effect on a person's phenotype and one which does not. The genotyping protocol is relatively inexpensive and uses standard molecular biology reagents and equipment.
ISSN:0021-9266
2157-6009
DOI:10.1080/00219266.2015.1007885