Sporulation of Gibberella zeae. IV. Role of the tricarboxylic-acid cycle in macroconidium production

In shake culture Gibberella seae sporulated poorly when glucose or other sugars were used singly as carbon sources. However, G. zeae sporulated one order of magnitude more when either acetate or a TCA-cycle intermediate was used singly as the carbon source. The continuous addition of catalytic amoun...

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Bibliographic Details
Published in:Mycologia 1979-07, Vol.71 (4), p.688-698
Main Authors: Huang, B.F, Dawson, R.F, Cappellini, R.A
Format: Article
Language:English
Subjects:
Acetates
Citrates
fungal diseases of plants
Gibberella
Glyoxylate cycle
Organic acids
Paper chromatography
plant health
plant pathogenic fungi
Sodium
Solvents
Spores
Sporulation
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Summary:In shake culture Gibberella seae sporulated poorly when glucose or other sugars were used singly as carbon sources. However, G. zeae sporulated one order of magnitude more when either acetate or a TCA-cycle intermediate was used singly as the carbon source. The continuous addition of catalytic amounts (totalling 8.3 × 10 -5 that of acetate) of TCA-cycle intermediates enhanced the sporulation of G. seae an additional two-to three-fold in acetate-shake culture. Likewise, the presence of 2 to 10% glucose in the acetate-shake culture had the same effect. Additional evidence implicating the TCA cycle in the sporulation of G. zeae was the fact that no TCA-cycle acids were found in the spent medium of sporulatting cultures. In the absence of trace amounts of TCA-cycle intermediate or glucose, an adequate supply of oxygen is important in the sporulation of G. zeae. It was hypothesized that the glyoxylate cycle may also be involved besides the TCA cycle continuously to provide oxaloacetate.
ISSN:0027-5514
1557-2536
DOI:10.1080/00275514.1979.12021063